IEB Institute of Experimental Botany, Czech Academy of Sciences
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Resources

Resources

Tools and datasets developed by the lab, made available for the community.

Internal tool · Gene-expression database
GeneViz

An internal browser for the lab’s gene-expression data — organisms, gene catalogs, and RNA-seq experiments (currently covering Nicotiana tabacum and the Arabidopsis thaliana reference genome), with genotype and cell-type annotations and dataset visualizations.

Availability

Accessible only from within the institute’s local network: geneviz.ueb.cas.cz/dashboard

Software / algorithmic pipeline
CATSNAP

Conserved AlTernative SpliciNg in Animals and Plants — an algorithmic pipeline for assessing the conservation of putative protein isoforms generated by alternative splicing. It employs a machine learning approach following a database search with the provided pair of protein sequences.

Availability

catsnap.cesnet.cz

Citation

Timofeyenko K, Kanavalau D, Alexiou P, Kalyna M, Růžička K (2023) Catsnap: a user-friendly algorithm for determining the conservation of protein variants reveals extensive parallelisms in the evolution of alternative splicing. New Phytologist, 238: 1722-1732. DOI: 10.1111/nph.18799

Dataset · N. tabacum L. cv Bright Yellow 2
Tobacco cell line BY-2 transcriptomic data

Seven biological replicates of Nicotiana tabacum L. cv. Bright Yellow (BY-2) cell culture samples cultured at auxin-supplemented conditions (ctrl) and three replicates cultured at auxin-starved (AF) conditions. Samples collected 48 hours after culture inoculation.

Availability

Raw sequencing data are available through GEO Series accession GSE160438 at the Gene Expression Omnibus.

Citation

Müller K, Dobrev PI, Pěnčík A, Hošek P, Vondráková Z, Filepová R, Malínská K, Brunoni F, Helusová L, Moravec T, Retzer K, Harant K, Novák O, Hoyerová K, Petrášek J (2021) DIOXYGENASE FOR AUXIN OXIDATION 1 catalyzes the oxidation of IAA amino acid conjugates. Plant Physiology, 187(1): 103-115. DOI: 10.1093/plphys/kiab242