|Head of the laboratory|
|RNDr. Jan Petrášek, Ph.D.|
Auxins and cytokinins in the regulation of cell division and elongation, cytoskeleton dynamics, regulation of auxin carrier’s dynamics and activity, hormonal crosstalk, polarity, plant development, auxin transport inhibitors.
|Ing. Milada Čovanová, Ph.D.
Role of Auxin Binding Protein 1 in the development of BY-2 tobacco cells and its involvement in the regulation of auxin level inside the cells.
|Ing. Petre Ivanov Dobrev, CSc.
Development of methods for the extraction, purification and determination of plant hormones using HPLC with on-line monitoring of UV-Vis, fluorescence, radioactivity and mass spectra. Metabolism of plant hormones, particularly cytokinins, in synchronized cell systems.
|RNDr. Nikoleta Dupľáková, Ph.D.
Non-canonical auxin transporters in Arabidopsis.
|Bc. Ing. Klára Hoyerová, Ph.D.
Molecular mechanisms of translocation of auxins and cytokinins across membranes in plant cells, metabolism and regulation of homeostasis of auxin and cytokinin and its mathematical modeling.
|Ing. Jana Jarošová, Ph.D.
Quantitative PCR expert; stress physiology; abiotic, biotic, and combined stresses.
|RNDr. Adriana Jelínková, Ph.D.
Identification of cytoskeletal and associated proteins that are involved in dynamics od endomembrane transport, polar PIN targetting and thus on cell polarity establishment.
|Mgr. Petr Klíma, Ph.D.
Transport of auxins and cytokinins across plant cell membranes, characterisation of carriers and hormonal metabolic regulation. Testing of inhibitors/compounds targetting these processes.
|Ing. Kateřina Malínská, Ph.D.
Fluorescence microscopy of plant plasma membrane proteins. Yeast as a tool to study auxin transport proteins.
|Ing. Václav Motyka, CSc.
Regulation of the metabolic inactivation (degradation and N-glucosylation) of cytokinins in relation to control of plant growth and development. Metabolism and biological functions of cis-zeatin-type cytokinins in plants. Effects of biotic and abiotic stresses on the contents and metabolism of phytohormones.
|Ing. Karel Müller, Ph.D.
Cloning of genes coding for tobacco auxin transporters, their expression and targeted silencing.
|Mgr. Kamil Růžička, Dr. rer. nat.
RNA processing in hormonal pathways. With particular focus on methylation of mRNA and splicing.
|RNDr. Radomíra Vaňková, CSc.
Regulation of the level of physiologically active cytokinins during the plant development by their reversible and irreversible glycosylation. The role of cytokinins and abscisic acid in plant response to abiotic stress and subsequent recovery.
|Prof. RNDr. Eva Zažímalová, CSc.
Mechanisms of transport of auxins and cytokinins across the plasma membrane; auxin carrier systems, auxin homeostasis and their regulation. Perception and transduction of auxin signal. Roles of these processes in the regulation of cell division and growth.
|Mgr. Tomáš Hluska, Ph.D.
Do cytokinin N-glucosides act as active cytokinins per se or do they first need to be hydrolyzed? Synthesis of N-glucosides by glucosyltransferases. Cytokinin biosynthesis, namely cis-zeatin, dihydrozeatin and aromatic cytokinins.
|Ing. Petr Hošek, Ph.D.
Computational modelling of auxin transport and cytokinin metabolism. And statistics.
|Ing. Eva Pokorná, Ph.D.
Metabolic pathways regulating the levels of bioactive cytokinins in plants.
|Mgr. Sylva Přerostová, PhD.
Stress physiology, role of plant hormones in abiotic stress responses, regulation of phytohormone homeostasis.
|Dr.nat.techn. Katarzyna Retzer, Ph.D.
Structural analysis of AUX1; auxin carrier regulated root growth adaptation.
|RNDr. Martina Laňková, Ph.D.
Mechanism of transport of plant hormone auxin into tobacco cells, characterisation of an auxin influx carrier and its inhibitors.
|Ing. Roberta Filepová
Media preparation, extraction of phytohormones, accumulation assays.
|RNDr. Alena Gaudinová
Analytical methods for phytohormones; determination of level and activity of the enzymes of cytokinin biosynthesis and metabolism.
|Mgr. Zuzana Vondráková
Transformation of tobacco and Arabidopsis suspension cultures, cultivation of plant material, sampling and sample preparation, RNA isolation.
|Mgr. Nikola Drážná – on maternity leave
Isolation of plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
|Bc. Vojtěch Knirsch
Cultivation of plant material, sampling and sample preparation, isolation of plant hormones, membrane integrity measurement.
Isolation of the plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
Isolation of the plant hormones auxins and cytokinins from plant tissues. Isolation of cytokininoxidase from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
Immunolocalizations, transformations, cultivation of plant material (in vitro cultures).
Ph.D. dissertation theme: Functional characterization of alternative splicing of auxin transporters and its role in plant development. In collaboration with CEITEC Masaryk University, Brno, Czech Republic. Supervisor: K. Růžička.
|Ing. Jozef Lacek
Ph.D. dissertation theme: Characterisation of factors participating on regulation of intracellular dynamics of auxin carriers.
Charles University, Faculty of Science, Department of Experimental Plant Biology. Since 2013. Supervisor: E. Zažímalová, advisors: M. Čovanová, K. Retzer
Ph.D. dissertation theme: Auxin sinks of the PIN5 transporter in plant development - new IAA degradation pathway and phenotypic consequences.
Charles University, Faculty of Science, Department of Experimental Plant Biology. Since 2020. Supervisor: Kamil Růžička. Advisor: Jan Petrášek.
|Mgr. Daniel Nedvěd
Ph.D. dissertation theme: Identification of interactions of auxin and cytokinin supported by mathematical modelling. Charles University, Faculty of Science, Department of Experimental Plant Biology. Since 2020. Supervisor: Klára Hoyerová, advisor: Jan Petrášek
|Mgr. Roman Skokan
Ph.D. dissertation theme: Evolution of auxin homeostasis mechanisms.
Charles University, Faculty of Science, Department of Experimental Plant Biology. Since 2014. Supervisor: J. Petrášek, advisor: K. Müller
Ph.D. dissertation theme: Role of alternative splicing in abscisic acid and brassinosteroid pathways. In collaboration with CEITEC Masaryk University, Brno, Czech Republic. Supervisor: K. Růžička.
Ph.D. dissertation theme: Modifications of adenine in RNAs and their role in plant development and auxin pathways. In collaboration with CEITEC Masaryk University, Brno, Czech Republic. Supervisor: K. Růžička.
|Bc. Vojtěch Schmidt, master student
Diploma thesis: The role of auxin in algal ancestors of land plants. Charles University, Faculty of Science, Department of Experimental Plant Biology. Supervisor: Jan Petrášek. Advisors: Roman Skokan, Stanislav Vosolsobě.
|Lenka Helusová, master student
Diploma thesis: Analysis of Gretchen Hagen 3 gene family on tobacco BY-2 plants and cell culture. Charles University, Faculty of Science, Department of Experimental Plant Biology. Supervisor: Karel Müller. Advisor: Jan Petrášek.
|Lenka Novotná, bachelor student
Diploma thesis: Mechanisms coordinating auxin metabolism and transport. Charles University, Faculty of Science, Department of Experimental Plant Biology. Supervisor: Jan Petrášek. Advisor: Karel Müller.