|Head of the laboratory|
|RNDr. Jan Petrášek, Ph.D.|
Auxins and cytokinins in the regulation of cell division and elongation, cytoskeleton dynamics, regulation of auxin carrier’s dynamics and activity, hormonal crosstalk, polarity, plant development, auxin transport inhibitors.
|Ing. Petre Ivanov Dobrev, CSc.
Development of methods for the extraction, purification and determination of plant hormones using HPLC with on-line monitoring of UV-Vis, fluorescence, radioactivity and mass spectra. Metabolism of plant hormones, particularly cytokinins, in synchronized cell systems.
|Bc. Ing. Klára Hoyerová, Ph.D.
Molecular mechanisms of translocation of auxins and cytokinins across membranes in plant cells, metabolism and regulation of homeostasis of auxin and cytokinin and its mathematical modeling.
|RNDr. Adriana Jelínková, Ph.D.
Identification of cytoskeletal and associated proteins that are involved in dynamics od endomembrane transport, polar PIN targetting and thus on cell polarity establishment.
|Ing. Miroslav Kamínek, CSc.
Mechanisms of metabolic regulation of cytokinin levels in plant cells, their function in control of plant development and exploitation in enhancement of plant productivity.
|Mgr. Petr Klíma, Ph.D.
Transport of auxins and cytokinins across plant cell membranes, characterisation of carriers and hormonal metabolic regulation. Testing of inhibitors/compounds targetting these processes.
|Ing. Kateřina Malínská, Ph.D.
Fluorescence microscopy of plant plasma membrane proteins. Yeast as a tool to study auxin transport proteins.
|Ing. Václav Motyka, CSc.
Regulation of the metabolic inactivation (degradation and N-glucosylation) of cytokinins in relation to control of plant growth and development. Metabolism and biological functions of cis-zeatin-type cytokinins in plants. Effects of biotic and abiotic stresses on the contents and metabolism of phytohormones.
|Ing. Karel Müller, Ph.D.
Cloning of genes coding for tobacco auxin transporters, their expression and targeted silencing.
|Mgr. Kamil Růžička, Dr. rer. nat.
RNA processing in hormonal pathways. With particular focus on methylation of mRNA and splicing.
|RNDr. Radomíra Vaňková, Ph.D.
Regulation of the level of physiologically active cytokinins during the plant development by their reversible and irreversible glycosylation. The role of cytokinins and abscisic acid in plant response to abiotic stress and subsequent recovery.
|Prof. RNDr. Eva Zažímalová, CSc.
Mechanisms of transport of auxins and cytokinins across the plasma membrane; auxin carrier systems, auxin homeostasis and their regulation. Perception and transduction of auxin signal. Roles of these processes in the regulation of cell division and growth.
|Ing. Milada Čovanová, Ph.D.
Role of Auxin Binding Protein 1 in the development of BY-2 tobacco cells and its involvement in the regulation of auxin level inside the cells.
|Mgr. Tomáš Hluska, Ph.D.
Do cytokinin N-glucosides act as active cytokinins per se or do they first need to be hydrolyzed? Synthesis of N-glucosides by glucosyltransferases. Cytokinin biosynthesis, namely cis-zeatin, dihydrozeatin and aromatic cytokinins.
|Ing. Petr Hošek, Ph.D.
Computational modelling of auxin transport and cytokinin metabolism. And statistics.
|Ing. Eva Pokorná, Ph.D.
Metabolic pathways regulating the levels of bioactive cytokinins in plants.
|Dr.nat.techn. Katarzyna Retzer, Ph.D.
Structural analysis of AUX1; auxin carrier regulated root growth adaptation.
|RNDr. Martina Laňková, Ph.D.
Mechanism of transport of plant hormone auxin into tobacco cells, characterisation of an auxin influx carrier and its inhibitors.
|Ing. Roberta Filepová
Media preparation, extraction of phytohormones, accumulation assays.
|Mgr. Markéta Fílová – on maternity leave
Transformation of tobacco and Arabidopsis suspension cultures.
|RNDr. Alena Gaudinová
Analytical methods for phytohormones; determination of level and activity of the enzymes of cytokinin biosynthesis and metabolism.
|Mgr. Zuzana Vondráková
Transformation of tobacco and Arabidopsis suspension cultures, cultivation of plant material, sampling and sample preparation, RNA isolation.
|Mgr. Nikola Drážná – on maternity leave
Isolation of plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
|Bc. Vojtěch Knirsch
Cultivation of plant material, sampling and sample preparation, isolation of plant hormones, membrane integrity measurement.
Isolation of the plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
Isolation of the plant hormones auxins and cytokinins from plant tissues. Isolation of cytokininoxidase from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
Ph.D. dissertation theme: Functional characterization of alternative splicing of auxin transporters and its role in plant development. In collaboration with CEITEC Masaryk University, Brno, Czech Republic. Supervisor: K. Růžička.
|Mgr. Barbara Kramná
Ph.D. dissertation theme: Strigolactone and phosphate cross-talk – its impact on the antioxidant system and plant physiological state.
Faculty of Science, Charles University in Prague. Since 2015. Supervisor: R. Vaňková
|Ing. Jozef Lacek
Ph.D. dissertation theme: Characterisation of factors participating on regulation of intracellular dynamics of auxin carriers.
Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2013. Supervisor: E. Zažímalová, advisors: M. Čovanová, K. Retzer
|Mgr. Sylva Přerostová
Ph.D. dissertation theme: Characterization of the role of cytokinins and abscisic acid during abiotic stress response.
Faculty of Science, Charles University in Prague. Since 2011. Supervisor: R. Vaňková
|Mgr. Roman Skokan
Ph.D. dissertation theme: Evolution of auxin homeostasis mechanisms.
Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2014. Supervisor: J. Petrášek, advisor: K. Müller
Ph.D. dissertation theme: Role of alternative splicing in abscisic acid and brassinosteroid pathways. In collaboration with CEITEC Masaryk University, Brno, Czech Republic. Supervisor: K. Růžička.
Ph.D. dissertation theme: Modifications of adenine in RNAs and their role in plant development and auxin pathways. In collaboration with CEITEC Masaryk University, Brno, Czech Republic. Supervisor: K. Růžička.
|Bc. Daniel Nedvěd
Diploma thesis: Mechanism of cytokinin transport across plasma membrane. Characterization of BY-2 cell lines overexpressing candidate cytokinin transporters. Supervisor: Assoc. Prof. Helena Ryšlavá (Charles University, Faculty of Science, Department of Biochemistry). Advisors: Klára Hoyerová, Petr Klíma