People

Head of the laboratory

RNDr. Jan Petrášek, Ph.D.

petrasek@ueb.cas.cz

Auxins and cytokinins in the regulation of cell division and elongation, cytoskeleton dynamics, regulation of auxin carrier’s dynamics and activity, hormonal crosstalk, polarity, plant development, auxin transport inhibitors.

 


Researchers

Ing. Petre Ivanov Dobrev, CSc.

dobrev@ueb.cas.cz

Development of methods for the extraction, purification and determination of plant hormones using HPLC with on-line monitoring of UV-Vis, fluorescence, radioactivity and mass spectra. Metabolism of plant hormones, particularly cytokinins, in synchronized cell systems.

 


Bc. Ing. Klára Hoyerová, Ph.D.

hoyerova@ueb.cas.cz

Molecular mechanisms of translocation of auxins and cytokinins across membranes in plant cells, metabolism and regulation of homeostasis of auxin and cytokinin and its mathematical modeling.

 


RNDr. Adriana Jelínková, Ph.D.

cernaa@ueb.cas.cz

Identification of cytoskeletal and associated proteins that are involved in dynamics od endomembrane transport, polar PIN targetting and thus on cell polarity establishment.

 


Ing. Miroslav Kamínek, CSc.

kaminek@ueb.cas.cz

Mechanisms of metabolic regulation of cytokinin levels in plant cells, their function in control of plant development and exploitation in enhancement of plant productivity.

 


Mgr. Petr Klíma, Ph.D.

klima@ueb.cas.cz

Transport of auxins and cytokinins across plant cell membranes, characterisation of carriers and hormonal metabolic regulation. Testing of inhibitors/compounds targetting these processes.

 


Ing. Kateřina Malínská, Ph.D.

malinska@ueb.cas.cz

Fluorescence microscopy of plant plasma membrane proteins. Yeast as a tool to study auxin transport proteins.

 


Ing. Václav Motyka, CSc.

motyka@ueb.cas.cz

Regulation of the metabolic inactivation (degradation and N-glucosylation) of cytokinins in relation to control of plant growth and development. Metabolism and biological functions of cis-zeatin-type cytokinins in plants. Effects of biotic and abiotic stresses on the contents and metabolism of phytohormones.


Ing. Karel Müller, Ph.D.

muller@ueb.cas.cz

Cloning of genes coding for tobacco auxin transporters, their expression and targeted silencing.

 


Mgr. Kamil Růžička, Dr. rer. nat.

ruzicka@ueb.cas.cz

 

 


Ing. Petr Skůpa, Ph.D.

skupa@ueb.cas.cz

Changes of phenotype of cell suspension in relation to the expression of transgenes affecting hormonal regulations in plant cells.

 


RNDr. Radomíra Vaňková, Ph.D.

vankova@ueb.cas.cz

Regulation of the level of physiologically active cytokinins during the plant development by their reversible and irreversible glycosylation. The role of cytokinins and abscisic acid in plant response to abiotic stress and subsequent recovery.


Prof. RNDr. Eva Zažímalová, CSc.

zazimalova@ueb.cas.cz

Mechanisms of transport of auxins and cytokinins across the plasma membrane; auxin carrier systems, auxin homeostasis and their regulation. Perception and transduction of auxin signal. Roles of these processes in the regulation of cell division and growth.


Postdoctoral fellows

Ing. Milada Čovanová, Ph.D.on maternity leave

covanova@ueb.cas.cz

Role of Auxin Binding Protein 1 in the development of BY-2 tobacco cells and its involvement in the regulation of auxin level inside the cells.

 


Ing. Petr Hošek, Ph.D.

hosek@ueb.cas.cz

Computational modelling of auxin transport and cytokinin metabolism. And statistics.

 


Dr.rer.nat. Katarzyna Retzer, Ph.D.

retzer@ueb.cas.cz

Structural analysis of AUX1; auxin carrier regulated root growth adaptation.

 


Ing. Eva Žižková, Ph.D.

zizkovae@ueb.cas.cz

Metabolic pathways regulating the levels of bioactive cytokinins in plants.

 


Research assistants

RNDr. Martina Laňková, Ph.D.

lankova@ueb.cas.cz

Mechanism of transport of plant hormone auxin into tobacco cells, characterisation of an auxin influx carrier and its inhibitors.

 


Research specialists

Ing. Roberta Filepová

filepova@ueb.cas.cz

Media preparation, extraction of phytohormones, accumulation assays.

 


Mgr. Markéta Fílováon maternity leave

filova@ueb.cas.cz

Transformation of tobacco and Arabidopsis suspension cultures.

 


RNDr. Alena Gaudinová

gaudinova@ueb.cas.cz

Analytical methods for phytohormones; determination of level and activity of the enzymes of cytokinin biosynthesis and metabolism.

 


Mgr. Zuzana Vondráková

vondrakovaz@ueb.cas.cz

Transformation of tobacco and Arabidopsis suspension cultures, cultivation of plant material, sampling and sample preparation, RNA isolation.

 


Technicians

Bc. Jiří Danko

danko@ueb.cas.cz

Cultivation of plant material, sampling and sample preparation, isolation of plant hormones, membrane integrity measurement.

 


Mgr. Nikola Drážná – on maternity leave

drazna@ueb.cas.cz

Isolation of plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).

 


Bc. Karolína Holečková

holeckova@ueb.cas.cz

Cultivation of plant material, sampling and sample preparation, isolation of plant hormones, membrane integrity measurement.

 


Bc. Vojtěch Knirsch

knirsch@ueb.cas.cz

Cultivation of plant material, sampling and sample preparation, isolation of plant hormones, membrane integrity measurement.

 


Eva Kobzová

kobzova@ueb.cas.cz

Isolation of the plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).

 


Marie Korecká

korecka@ueb.cas.cz

Isolation of the plant hormones auxins and cytokinins from plant tissues. Isolation of cytokininoxidase from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).

 


PhD. students

Mgr. Matouš Glanc

matous.glanc@ist.ac.at

Ph.D. dissertation theme: Mechanisms of polarity re-establishment in post-cytokinetic plant cells. Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2014.

Supervisor: J. Friml (IST, Klosterneuburg, Austria), advisors: E. Zažímalová, M. Fendrych


Mgr. Barbara Kramná

kramna@ueb.cas.cz

Ph.D. dissertation theme: Strigolactone and phosphate cross-talk – its impact on the antioxidant system and plant physiological state. Faculty of Science, Charles University in Prague. Since 2015.

Supervisor: R. Vaňková


Ing. Jozef Lacek

lacek@ueb.cas.cz

Ph.D. dissertation theme: Characterisation of factors participating on regulation of intracellular dynamics of auxin carriers. Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2013.

Supervisor: E. Zažímalová, advisors: M. Čovanová, K. Retzer


Mgr. Sylva Přerostová

prerostova@ueb.cas.cz

Ph.D. dissertation theme: Characterization of the role of cytokinins and abscisic acid during abiotic stress response. Faculty of Science, Charles University in Prague. Since 2011.

Supervisor: R. Vaňková


Mgr. Roman Skokan

letajici.kiwi@seznam.cz

Ph.D. dissertation theme: Evolution of auxin homeostasis mechanisms. Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2014.

Supervisor: J. Petrášek, advisor: K. Müller


Bachelor students

Daniel Nedvěd